丙烯酰胺对人白血病细胞HPRT位点的影响

doi:10.3969/j.issn.1004-616x.2000.01.005

Carcinogenesis, Teratogenesis & Mutagenesis ›› 2000, Vol. 12 ›› Issue (1): 15-017.doi: 10.3969/j.issn.1004-616x.2000.01.005

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EFFECTS OF ACRYLAMIDE ON HPRT GENE OF HUMAN PROMYELOCYTIC LEUKEMIA CELLS

LIU Sheng-xue, CAO Jia , AN Hui, ZHOU Zi-yuan, SHUN Hua-min

Department of Molecular Toxicology, Third Military Medical University, Chongqing 　400038, China

Received:1999-04-04 Revised:1999-07-04 Online:2000-01-30 Published:2000-01-30

Abstract

Abstract: Purpose : To investigate the effect s of Acrylamide (AA) on the HPRT gene of human promyelocytic leukemia cells. Methods : HL - 60 cells wre infected with AA with different concent rations and put into single -cell wells at different time phases. Then the numbers of positive wells were counted and the cell plating efficiency , cloning efficiency and mutation f requency were determined. Results : the cell mutation f requency was positively correlated to the cocent ration. When the dose of AA rose up to the top (700μg/ ml) , there was clear mutagenesis on HPRT locus. Meanwhile the cloning efficiency increased slowly to follow increasing of doses ,and this pheomenon might be related with the st ronger toxicity of AA. Conclusion : The mutagenesis of AA was nferior.

Key words: acrylamide, HPRT gene, mutagenesis, HL - 60 cell

CLC Number:

LIU Sheng-xue, CAO Jia , AN Hui, ZHOU Zi-yuan, SHUN Hua-min. EFFECTS OF ACRYLAMIDE ON HPRT GENE OF HUMAN PROMYELOCYTIC LEUKEMIA CELLS[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2000, 12(1): 15-017.

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EFFECTS OF ACRYLAMIDE ON HPRT GENE OF HUMAN PROMYELOCYTIC LEUKEMIA CELLS

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